Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to PhenoSense GT®

CPT: 87536. If reflex testing is performed, concomitant CPT codes/charges will apply.
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Synonyms

  • HIV-1 Plasma Viremia
  • HIV-1 RNA by Real-time (RT) PCR, Quantitative
  • Reflex to PhenoSense GT®
  • Viral Load

Expected Turnaround Time

17 - 25 days



Related Documents

For more information, please view the literature below.

HIV Specimen Collection Guide

HIV-1 Lifecycle, Drugs, and Susceptibility Assays


    Specimen Requirements


    Specimen

    Plasma, frozen


    Volume

    6 mL


    Minimum Volume

    4 mL (Note: This volume does not allow for repeat testing.)


    Container

    Ship in two or more screw-cap polypropylene frozen transport tubes.


    Collection

    Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Remove plasma, transfer plasma to screw-cap polypropylene transport tubes (not "pop-top" or "snap-cap"), and freeze. Ship frozen (preferred). Do not ship frozen in PPT™. If PPT™ is used to collect plasma, specimen must be transferred to screw-cap tubes prior to freezing to avoid false elevation of low viral load samples. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


    Storage Instructions

    Freeze. Separate frozen sample required for PhenoSenseGT® portion of the test. Frozen sample is preferred for the viral load assay but is also stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.


    Causes for Rejection

    Hemolysis; green-top (heparin) tube; specimen received in "pop-top" or "snap-cap" tube; quantity not sufficient for analysis; frozen PPT™


    Test Details


    Use

    Detect and quantitate HIV-1 in plasma with reflex to PhenoSenseGT® (at an additional charge) if viral load is >500 copies/mL.


    Limitations

    Assay range: 20−10,000,000 copies/mL


    Methodology

    Cobas® AmpliPrep/Cobas® TaqMan® HIV-1 Test, Version 2.0


    LOINC® Map

    Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
    550450 RNA, PCR (NonGraph) rfx/PSGT 550445 HIV-1 RNA by PCR copies/mL 20447-9
    550450 RNA, PCR (NonGraph) rfx/PSGT 550446 log10 HIV-1 RNA log10copy/mL 29541-0
    550450 RNA, PCR (NonGraph) rfx/PSGT 550451 PhenoSense GT(R) N/A
    Reflex Table for PhenoSense GT(R)
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551121 Reflex to PhenoSense GT(R) 551601 PhenoSense GT(R) PDF 51969-4
    Reflex Table for PhenoSense GT(R)
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551121 Reflex to PhenoSense GT(R) 551602 PhenoSense(R) PDF 51969-4
    Reflex Table for PhenoSense GT(R)
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551121 Reflex to PhenoSense GT(R) 551603 GeneSeq(R) PDF 51969-4
    Reflex Table for PhenoSense GT(R)
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551121 Reflex to PhenoSense GT(R) 551604 PSGT-Net Assessment 19146-0
    Reflex Table for PhenoSense GT(R)
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551121 Reflex to PhenoSense GT(R) 551605 PSGT-PhenoSense(R) Results 49665-3
    Reflex Table for PhenoSense GT(R)
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551121 Reflex to PhenoSense GT(R) 551608 PSGT-GeneSeq(R) Results 53798-5
    Reflex Table for PhenoSense GT(R)
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551121 Reflex to PhenoSense GT(R) 551609 PSGT-Net Assessment Interp. 77202-0
    Reflex Table for PhenoSense GT(R)
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551121 Reflex to PhenoSense GT(R) 551617 PSGT-PhenoSense(R) Interp. 62364-5
    Reflex Table for PhenoSense GT(R)
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551121 Reflex to PhenoSense GT(R) 551618 PSGT-GeneSeq(R) Interp. 62364-5

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