Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical)

TEST: 550430
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CPT: 87536
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Synonyms

  • HIV-1 Plasma Viremia
  • HIV-1 RNA by Real-time PCR, Quantitative

Special Instructions

This procedure does not provide serial monitoring; it is intended for one-time use only. If serial monitoring is required, please order test 550420.

Expected Turnaround Time

3 - 4 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

For more information, please view the literature below.

HIV Specimen Collection Guide

Human Immunodeficiency Virus 1/O/2 Cascade: Fourth-generation Antigen/Antibody Detection

Specimen Requirements

Specimen

Plasma

Volume

3.5 mL

Minimum Volume

1.1 mL (Note: This volume does not allow for repeat testing.)

Container

Lavender-top (EDTA) tube or plasma preparation tube (PPT™)

Collection

Centrifuge sample within 24 hours of collection. Transfer plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.

Storage Instructions

Freeze (preferred) or refrigerate. Stable at room temperature for 24 hours or refrigerated for six days. Stable frozen for six weeks.

Causes for Rejection

Incorrect anticoagulant; PPT™ frozen and/or not centrifuged

Test Details

Use

Detect and quantitate HIV-1 in plasma

Limitations

Assay range: 20−10,000,000 copies/mL

Methodology

Cobas® HIV

References

Wirden M, Larrouy L, Mahjoub N, et al. Multicenter comparison of the new Cobas 6800 system with Cobas Ampliprep/Cobas TaqMan and Abbott RealTime for the quantification of HIV, HBV and HCV viral load. J Clin Virol. 2017 Nov;96:49-53.28950186

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550430 RNA, Real Time PCR (Non-Graph) 20447-9 550413 HIV-1 RNA by PCR copies/mL 20447-9
550430 RNA, Real Time PCR (Non-Graph) 20447-9 550414 log10 HIV-1 RNA log10copy/mL 29541-0

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