Human Immunodeficiency Virus 1 (HIV-1) PhenoSense GT® (Monogram® Phenotype/Genotype)

TEST: 551690
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CPT: 87900; 87901; 87903; 87904(x12)
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Synonyms

  • HIV-1 Resistance
  • PhenoSense GT®

Special Instructions

A separate frozen specimen is required for each frozen test ordered. This assay is a combined assay in which HIV genotype and HIV phenotype is performed.

Expected Turnaround Time

16 - 22 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Related Documents

For more information, please view the literature below.

HIV Specimen Collection Guide

HIV-1 Lifecycle, Drugs, and Susceptibility Assays

Supporting Individualized HIV Treatment Decisions

Specimen Requirements

Specimen

Plasma, frozen

Volume

3 mL

Container

Lavender-top (EDTA) or plasma preparation tube (PPT™)

Collection

Collect specimen in two lavender-top (EDTA) tubes or two PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more polypropylene screw-capped tube(s) (not "pop-top" or "snap-cap"), and freeze. Ship specimen frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze

Causes for Rejection

PPT™ not centrifuged; quantity not sufficient for analysis; heparinized plasma; nonfrozen specimen; specimen exposed to repeated freeze/thaw cycles; specimen received in “pop-top” or “snap-cap” tube

Test Details

Use

Determines viral phenotype and genotype resistance to three classes of commonly prescribed antiretroviral drugs: nucleoside reverse transcriptase inhibitors (NRTI), nonnucleoside reverse transcriptase inhibitors (NNRTI), and protease inhibitors (PI). Viral replication capacity is also included.

Limitations

This procedure should be used for patients with documented HIV-1 infection and viral loads ≥500 copies/mL.

Methodology

Phenotype: polymerase chain reaction (PCR) amplification and viral culture; genotype: polymerase chain reaction (PCR) and DNA sequencing. PhenoSense® and PhenoSense GT® are trademarks of Monogram Biosciences®.

References

Baxter JD, Mayers DL, Wentworth DN, et al. CPCRA 046 Study Team. A pilot study of the short-term effects of antiretroviral management based on plasma genotypic antiretroviral resistance testing (GART) in patients failing antiretroviral therapy. Sixth Conference on Retroviruses and Opportunistic Infections, Chicago, Ill, 1999. Abstract LB8.
Boden D, Hurley A, Zhang L, et al. HIV-1 drug resistance in newly infected individuals. JAMA. 1999; 282(12):1135-1141. 10501116
Cohen C, Hunt S, Sension M, et al. Phenotypic resistance testing significantly improves response to therapy (Tx): A randomized trial (VIRA3001). Seventh Conference on Retroviruses and Opportunistic Infections, San Francisco, Calif: 2000. Abstract 237.
Durant J, Clevenbergh P, Halfon P, et al. Drug-resistance genotyping in HIV-1 therapy: The VIRADAPT randomized controlled trial. Lancet. 1999; 353(9171):2195-2199. Erratum: Lancet. 1999; 354(9184):1128. 10392984
Little SJ, Daar ES, D'Aquila RT, et al. Reduced antiretroviral drug susceptibility among patients with primary HIV infection. JAMA. 1999; 282(12):1142-1149. 10501117
Panel on Antiretroviral Guidelines for Adults and Adolescents. Guidelines for the use of antiretroviral agents in HIV-1-infected adults and adolescents. Department of Health and Human Services. Washington, DC: Department of Health and Human Services. January 28, 2000. Available at http://aidsinfo.nih.gov/ContentFiles/AdultandAdolescentGL.pdf. Accessed February 10, 2012.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
551690 PhenoSense GT (R) 551601 PhenoSense GT(R) PDF 51969-4
551690 PhenoSense GT (R) 551602 PhenoSense(R) PDF 51969-4
551690 PhenoSense GT (R) 551603 GeneSeq(R) PDF 51969-4
551690 PhenoSense GT (R) 551604 PSGT-Net Assessment 19146-0
551690 PhenoSense GT (R) 551605 PSGT-PhenoSense(R) Results 49665-3
551690 PhenoSense GT (R) 551608 PSGT-GeneSeq(R) Results 53798-5
551690 PhenoSense GT (R) 551609 PSGT-Net Assessment Interp. 77202-0
551690 PhenoSense GT (R) 551617 PSGT-PhenoSense(R) Interp. 62364-5
551690 PhenoSense GT (R) 551618 PSGT-GeneSeq(R) Interp. 62364-5

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