Human Immunodeficiency Virus 1 (HIV-1) PhenoSense GT® Plus Integrase (Monogram® Phenotype + Genotype)

TEST: 551920
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CPT: 87900; 87901; 87903; 87904(x16); 87906
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Synonyms

  • HIV Resistance
  • PhenoSense GT® Plus Integrase

Test Includes

This assay is a combined assay in which HIV resistance phenotype and HIV resistance genotype is performed.

Special Instructions

A separate frozen specimen is required for each frozen test ordered.

Expected Turnaround Time

19 - 23 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Related Documents

For more information, please view the literature below.

HIV Specimen Collection Guide

HIV-1 Lifecycle, Drugs, and Susceptibility Assays

PhenoSense GT® Plus Integrase

    Specimen Requirements

    Specimen

    Plasma, frozen

    Volume

    5 mL

    Collection

    Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more polypropylene screw-capped tube(s) (not "pop-top" or "snap-cap") and freeze. Ship specimen frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

    Storage Instructions

    Freeze.

    Causes for Rejection

    PPT™ not centrifuged; quantity not sufficient for analysis; heparinized plasma; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles; specimen received in "pop-top" or "snap-cap" tube

    Test Details

    Use

    Determines viral phenotype and genotype resistance to four classes of commonly prescribed antiretroviral drugs: nucleoside reverse transcriptase inhibitors (NRTI), nonnucleoside reverse transcriptase inhibitors (NNRTI), protease inhibitors (PI), and integrase inhibitors (INI).

    Limitations

    This procedure should be used for patients with documented HIV-1 infection and viral loads ≥500 copies/mL. If there is insufficient virus to produce results, HIV-1 RNA quantitation will be performed to confirm viral load, and this procedure will be billed at the account's prevailing fee.

    Methodology

    Phenotype: polymerase chain reaction (PCR) amplification and viral culture; Genotype: polymerase chain reaction (PCR) and DNA sequencing. PhenoSense® and PhenoSense GT® are trademarks of Monogram Biosciences®.

    References

    Boyd SD, Maldarelli F, Sereti I, et al. Transmitted raltegravir resistance in an HIV-1 CRF_AG-infected patient. Antivir Ther. 2011; 16(2):257-261. 21447876
    Cohen C, Hunt S, Sension M, et al. Phenotypic resistance testing significantly improves response to therapy (Tx): A randomized trial (VIRA3001), San Francisco, Calif: Seventh Conference on Retroviruses and Opportunistic Infections. 2000. Abstract 237.
    Durant J, Clevenbergh P, Halfon P, et al. Drug-resistance genotyping in HIV-1 therapy: The VIRADAPT randomized controlled trial. Lancet. 1999 Jun 26; 353(9171):2195-2199. Erratum: Lancet. 1999; 354(9184):1128. 10392984
    Panel on Antiretroviral Guidelines for Adults and Adolescents. Guidelines for the use of antiretroviral agents in HIV-1-infected adults and adolescents. Department of Health and Human Services. Washington, DC: Department of Health and Human Services. January 28, 2000. Available at http://aidsinfo.nih.gov/ContentFiles/AdultandAdolescentGL.pdf. Accessed February 10, 2012.
    Parkin N, Chappey C, Maroldo L, Bates M, Hellmann NS, Petropoulos CJ. Phenotypic and genotypic HIV-1 drug resistance assays provide complementary information. J Acquir Immune Defic Syndr. 2002 Oct 1; 31(2):128-136. 12394790
    Walworth CM, Ward D, Napolitano L, et al. Optimized antiretroviral drug selection achieves rapid and sustained supression of viral replication, despite transmission of HIV-1 exhibiting resistance to 5-drug classes. International Workshop on HIV and Hepatitis Virus. June 5-9, 2012, Stiges, Spain.
    Young B, Fransen S, Greenberg KS, et al. Transmission of integrase strand-transfer inhibitor multidrug-resistant HIV-1: Case report and response to raltegravir-containing antiretroviral therapy. Antivir Ther. 2011; 16(2):253-256. 21447875

    LOINC® Map

    Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
    551920 PhenoSenseGT(R) plus Integrase 551921 PSGT plus Integrase PDF 51969-4
    551920 PhenoSenseGT(R) plus Integrase 551922 PSGT+IN Net Assessment 83326-9
    551920 PhenoSenseGT(R) plus Integrase 551923 PSGT+IN Phenotype Results 49665-3
    551920 PhenoSenseGT(R) plus Integrase 551924 PSGT+IN Genotype Results 80689-3
    551920 PhenoSenseGT(R) plus Integrase 551925 PSGT+IN Interpretation 77202-0
    Reflex Table for PSGT+IN Net Assessment
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 550504 Quant, RNA PCR 550497 HIV-1 RNA by PCR copies/mL 20447-9
    Reflex Table for PSGT+IN Net Assessment
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 550504 Quant, RNA PCR 550498 log10 HIV-1 RNA log10copy/mL 29541-0

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