Human Immunodeficiency Virus 1 & 2 (HIV-1/HIV-2), Qualitative, RNA

TEST: 139825
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CPT: 87535; 87538
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Expected Turnaround Time

2 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

For more information, please view the literature below.

HIV Screening and Diagnosis White Paper

Specimen Requirements

Specimen

Plasma or serum

Volume

1 mL

Minimum Volume

650 µL

Container

Lavender-top (EDTA) tube, BD SST tube, BD Vacutainer PPT plasma preparation tube

Collection

Collect specimen in lavender-top (EDTA), gel-barrier (SST tube) or a PPT plasma preparation tube. Specimens centrifugation within 24 hours of collection. Transfer serum or plasma to a polypropylene screw-cap tube for shipment.

Ship frozen.

Storage Instructions

Freeze (preferred) or refrigerate.

Stability Requirements

Temperature

Period

Refrigerated

7 days

Frozen

6 weeks

Freeze/thaw cycles

Stable x3

Note: Stability provided by manufacturer or literature reference.

Causes for Rejection

Specimen grossly hemolyzed; specimen received at room temperature

Test Details

Use

This test is intended for use as an aid in the diagnosis of HIV-1 or HIV-2 infection, including acute or primary infection. Presence of HIV-1 or HIV-2 RNA in the plasma of patients without antibodies to HIV-1 or HIV-2 is indicative of acute or primary HIV-1 or HIV-2 infection. This assay may also be used as an additional test, when it is reactive, to confirm HIV-1 infection in an individual whose specimen is repeatedly reactive for HIV-1 and/or HIV-2 antibodies. This assay is not intended for use in donor testing.

Methodology

RT-PCR

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
139825 HIV-1/HIV-2 Qualitative RNA 96557-4 139826 HIV-1 RNA 25835-0
139825 HIV-1/HIV-2 Qualitative RNA 96557-4 139827 HIV-2 RNA 69353-1

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The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf