Legionella pneumophila Urinary Antigen

CPT: 87449
Updated on 11/15/2024
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Expected Turnaround Time

1 - 3 days



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Specimen Requirements


Specimen

Urine


Volume

5 mL


Container

Sterile screw-cap container


Storage Instructions

Refrigerate.


Stability Requirements

TemperaturePeriod
Refrigerated

Up to 14 days from collection 

(stability provided by manufacturer or literature reference)

TemperaturePeriod
Refrigerated

Up to 14 days from collection 

(stability provided by manufacturer or literature reference)


Causes for Rejection

Swab specimen; inappropriate specimen transport device; unlabeled specimen or name discrepancy between specimen and request label; nonurine specimen


Test Details


Use

This test is used to confirm the diagnosis of Legionella pneumophila serogroup 1 infection.

Confirm the diagnosis of Legionella pneumophila serogroup 1 infection

This test is used to confirm the diagnosis of Legionella pneumophila serogroup 1 infection.


Limitations

This test will not detect species or serogroups other than Legionella pneumophila serogroup 1.


Methodology

Enzyme immunoassay (EIA)


Reference Interval

No Legionella pneumophila serogroup 1 antigen detected


Additional Information

Legionella pneumophila has been estimated to cause 80% to 85% of Legionella infections, with serogroup 1 accounting for most of these. Current methods of diagnosis for Legionella infection include culture and DFA. All of these other tests require a high quality respiratory specimen to perform this test and, unfortunately, many patients with Legionella infection do not produce sputum. A number of investigators have shown that a specific soluble antigen was present in the urine of patients with Legionnaires' disease, which can be detected both early and late in the disease.


References

Berdal PP, Farshy CE, Feelley JC. Detection of Legionella pneumophila antigen in urine by enzyme-linked immunospecific assay. J Clin Microbiol. 1979 May; 9(5):575-578. 383744
Kohler RB, Zimmerman SE, Wilson E, et al. Rapid immunoassay diagnosis of Legionnaires' disease: Detection and partial characterization of urinary antigen. Ann Intern Med. 1981 May; 94(5):601-605. 7235392
Reingold AL, Thomason BM, Brake BJ, Thacker L, Wilkinson HW, Kuritsky JN. Legionella pneumonia in the United States: The distribution of serogroups and species causing human illness. J Infect Dis. 1984 May; 149(5):819. 6726009

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