Testosterone, Total, Women, Children, and Hypogonadal Males, LC/MS-MS

Serum

0.8 mL

0.6 mL (Note: This volume does not allow for repeat testing.)

Red-top tube or gel-barrier tube

This assay provides the sensitivity and specificity required for the assessment of the low testosterone levels found in women, children, adolescents, and hypogonadal men.¹

Drugs, including androgens and steroids, can decrease testosterone levels. Men with advanced prostate cancer often receive drugs that lower testosterone levels. Women receiving estrogen may have increased testosterone levels. Anticonvulsants, barbiturates, and clomiphene can cause testosterone levels to rise.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Liquid chromatography/tandem mass spectrometry (LC/MS-MS)

See table.

Testosterone is the principal androgen in men.^2,3 The production of testosterone by the male testes is stimulated by luteinizing hormone (LH), which is produced by the pituitary. LH secretion is, in turn, inhibited through a negative feedback loop by increased concentrations of testosterone and its metabolites. Most of the testosterone in males is produced by the Leydig cells of the testes and is secreted into the seminiferous tubule, where it is complexed to a protein made by the Sertoli cells. This results in the high local levels of testosterone that are required for normal sperm production.

Diminished testosterone production is one of many potential causes of infertility in males.^3,4 Low testosterone concentrations can be caused by testicular failure (primary hypogonadism) or inadequate stimulation by pituitary gonadotropins (secondary hypogonadism). Several congenital conditions (ie, Klinefelter syndrome, Kallmann syndrome, Prader-Willi syndrome) can result in decreased testosterone production. Testosterone can also be diminished as the result of testicular damage caused by alcoholism, physical injury, viral diseases (eg, mumps), and in certain malignancies.

The adult male reference range for testosterone was established by Travison and coworkers through an epidemiologic study that included men from different geographic regions of the United States and Europe.⁵ Testosterone measurment was harmonized to the Center for Disease Control reference method.⁵ The reference population included only men younger than 40 years of age who had a BMI less than 30.

Significant physiological changes occur in men as they age, in part due to a gradual decline in testosterone levels.^6,7 It is generally accepted that the principal cause of this age-related decrease in testosterone production is testicular failure, although diminished gonadotropin production may play a role.⁸ By 75 years of age, the average male testosterone drops to 65% of average level in young adults. "Andropause" is a term that has been used to refer to the constellation of symptoms associated with age-related decline in testosterone production in men.^8.9 Since men with hypogonadism often have high SHBG levels, the measurement of free (or bioavailable) testosterone has been advocated when total testosterone levels are normal in men with symptoms of androgen deficiency.⁴

Much smaller amounts of testosterone and dihydrotestosterone are produced in women than in men.^3,4 Weaker adrenal androgens and ovarian precursor molecules, including androstenedione, DHEA, and DHEA sulfate, can have significant androgenic effects in women. The ovary and adrenal glands produce some testosterone, but the majority of the testosterone in women is derived from the peripheral conversion of other steroids. Often, the first sign of testosterone excess in women is the development of male pattern hair growth, which is referred to as hirsutism.^3,10,11 It should be noted that some women experience hair growth similar to that caused by increased testosterone due to racial or genetic causes and not due to excessive androgens. Measurement of testosterone may help to distinguish racial or genetic causes of hirsutism from abnormal pathology, particularly in women with mixed ethnic backgrounds. Women with more excessive testosterone levels may also experience virilization with symptoms that include increased muscle mass, redistribution of body fat, enlargement of the clitoris, deepening of the voice, and acne and increased perspiration. These women can also suffer from androgenic alopecia, the female equivalent of male pattern baldness.

Many women with slowly progressive androgenic symptoms are diagnosed as having polycystic ovary syndrome (PCOS).^11-14 PCOS is relatively common, affecting approximately 6% of women of reproductive age.² Women with this complex syndrome experience symptoms of androgen excess associated with menstrual abnormalities and infertility. Chronic anovulation experienced by patients with PCOS increases their risk of developing endometrial cancer. Women with PCOS are often overweight and are likely to suffer from insulin resistance, putting them at increased risk for developing type 2 diabetes mellitus.^2,12 Obesity and insulin resistance can result in acanthosis nigricans, a skin condition that is characterized by hyperpigmented, velvety plaques of body folds.² Lipid abnormalities, including decreased high-density lipoprotein cholesterol levels and elevated triglyceride levels, as well as impaired fibrinolysis, are seen in women with PCOS.^12,14 Cardiovascular disease is more prevalent, and women with PCOS have a significantly increased risk for myocardial infarction.^12,14