China Bioanalysis Forum 2024 -- An antisense oligonucleotide (ASO) is a single-stranded ribonucleotide therapeutic, typically 16-20 nucleotides long, designed to bind complementary to the target RNA via Watson-Crick base pairing. Determination of oligonucleotides in biological tissues yields information which assists investigators in pharmacokinetic studies and may help to avoid potential organ toxicity. Compound A, a novel unconjugated ASO was designed to treat chronic hepatitis B for a functional cure. Through a global development strategy, Compound A is advancing rapidly towards the goal of HBV cure. To support a toxicity study of Compound A, a robust method was set up for the quantification of Compound A in different types of tissue homogenate, including kidney, liver, spleen, placenta, milk and in different species including mouse, monkey and rabbit.

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