Human Immunodeficiency Virus 1 (HIV-1) GenoSure® Integrase

TEST: 551871
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CPT: Call 800-222-7566, ext 6-8400, for coding.
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Synonyms

  • HIV-1 Genotype
  • Resistance Testing
  • Retroviral Genotype

Special Instructions

Submit a separate frozen specimen for each test requested.

Expected Turnaround Time

9 - 15 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

For more information, please view the literature below.

HIV Specimen Collection Guide

HIV-1 Lifecycle, Drugs, and Susceptibility Assays

Supporting Individualized HIV Treatment Decisions

Specimen Requirements

Specimen

Plasma, frozen

Volume

5 mL

Container

Lavender-top (EDTA) or plasma preparation tube (PPT™)

Collection

Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Snap-cap or pop-top tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at -20°C. Do not place samples on dry ice until completely frozen. Ship specimens frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze.

Causes for Rejection

Use of green-top (heparin) tube; hemolysis; PPT™ not centrifuged; specimen at room temperature for more than 24 hours

Test Details

Use

Detect resistance of HIV-1 to antiretroviral drugs only in the integrase class.

Limitations

This procedure may not be successful when the HIV viral load is <500 copies/mL plasma. If there is insufficient virus to produce results, HIV-1 RNA quantitation will be performed to confirm viral load, and the procedure will be billed at the account's prevailing fee.

Methodology

Polymerase chain reaction (PCR) amplification and DNA sequencing

References

Cooper DA, Steigbigel RT, Gatell, JM, et al. Subgroup and resistance analyses of raltegravir for resistant HIV-1 infection. N Engl J Med. 2008 Jul 24; 359(4):355-365. 18650513
Evering T, Markowitz M. HIV integrase inhibitors.PRN Notebook. 2008; 13:1-9.
Hatano H, Lampiris H, Gupta S, et al. Long-term evolution of integrase resistance during failure of integrase inhibitor-based ART. Abstract 621, presented at 16th Conference on Retroviruses and Opportunistic Infections (CROI), February 2009, Montreal, Canada.
Malet I, Delelis O, Valantin MA, et al. Mutations associated with failure of raltegravir treatment affect integrase sensitivity to the inhibitor in vitro. Antimicrob Agents Chemother. 2008 Apr; 52(4):1351-1358. 18227187

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
551871 GenoSure(R) Integrase 61199-6 551932 PDF 11502-2
551871 GenoSure(R) Integrase 61199-6 551933 HIV GenoSure(R) Integrase 93047-9
551871 GenoSure(R) Integrase 61199-6 551934 HIV GenoSure(R) Integrase N/A
Reflex Table for HIV GenoSure(R) Integrase
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551319 Quant, RNA PCR 550415 HIV-1 RNA by PCR copies/mL 20447-9
Reflex Table for HIV GenoSure(R) Integrase
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551319 Quant, RNA PCR 550416 log10 HIV-1 RNA log10copy/mL 29541-0
Reflex Table for HIV GenoSure(R) Integrase
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551913 HIV GenoSure(R) Integrase 551913 HIV GenoSure(R) Integrase N/A
Reflex Table for HIV GenoSure(R) Integrase
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551914 HIV GenoSure(R) Integrase I... 551914 HIV GenoSure(R) Integrase I... N/A

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