Immunofixation (IFE), Serum

TEST: 001685
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CPT: 82784(x3) (IgG, IgA, IgM); 86334 (immunofixation electrophoresis)
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Synonyms

  • Electrophoresis, Serum, IFE
  • Immunoelectrophoresis, Serum

Test Includes

Quantitation of immunoglobulins IgA, IgG, and IgM; IFE interpretation of monoclonal heavy- (IgG, IgA, IgM, IgD, IgE) and light-chain (kappa, Lambda) patterns, if present in the serum

Special Instructions

State patient's age and clinical diagnosis on the test request form.

Expected Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Related Documents

For more information, please view the literature below.

Published Plasma Cell Dyscrasias Selection Table

Specimen Requirements

Specimen

Serum

Volume

2 mL

Minimum Volume

1 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube or gel-barrier tube

Collection

Separate serum from red cells.

Storage Instructions

Room temperature

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Patient Preparation

Overnight fasting is preferred, but not required.

Causes for Rejection

Plasma sample

Test Details

Use

Detect and identify monoclonal immunoglobulin gammopathies

Methodology

Electrophoresis followed by immunodiffusion against monospecific antisera to immunoglobulin and individual heavy and light chains (IFE); turbidimetric quantitation of IgA, IgG, and IgM

Reference Interval

Normal pattern

Additional Information

Immunofixation electrophoresis of serum or urine is most often ordered to evaluate a monoclonal globulin detected in a protein electrophoresis or to delineate a possible lymphoproliferative process, particularly myeloma. This procedure will characterize the specific light and heavy chain components of a monoclonal protein.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
001685 Immunofixation, Serum 34550-4 001795 Immunofixation Result, Serum 14895-7
001685 Immunofixation, Serum 34550-4 001776 Immunoglobulin G, Qn, Serum mg/dL 2465-3
001685 Immunofixation, Serum 34550-4 001784 Immunoglobulin A, Qn, Serum mg/dL 2458-8
001685 Immunofixation, Serum 34550-4 001792 Immunoglobulin M, Qn, Serum mg/dL 2472-9

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