Reaching the next level of sensitivity in oligonucleotide bioanalysis: a hybridization-based method on Quanterix Simoa®

Labcorp Bioanalytical Services webinar entitled, "Reaching the next level of sensitivity in oligonucleotide bioanalysis: a hybridization-based method on Quanterix Simoa®" presents a case study where a hybridization assay was developed on the Quanterix Simoa® for oligonucleotides. Challenges faced during method establishment on this ultra-sensitive immunoassay technology are discussed, such as labeling of paramagnetic beads, assay reproducibility and precision, stability of conjugated paramagnetic beads, along with approaches taken to overcome and/or mitigate. To evaluate the gains in sensitivity, the Quanterix Simoa® assay was compared to a more conventional hybridization assay approach using electrochemiluminescence (ECL) for detection of the same oligonucleotide therapeutic and the limits of quantitation achieved.